NET1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-40800
Key Product Details
Species Reactivity
Validated:
Human
Predicted:
Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-DKLEYLDEKQRDPR corresponding to internal region according to NP_001040625.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_001040625.1 and NP_005854.2).
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for NET1 Antibody
![Immunohistochemistry-Paraffin: NET1 Antibody [NB100-40800]](https://resources.bio-techne.com/images/products/NET1-Antibody-Immunohistochemistry-Paraffin-NB100-40800-img0002.jpg "Immunohistochemistry-Paraffin: NET1 Antibody [NB100-40800]")
Immunohistochemistry-Paraffin: NET1 Antibody [NB100-40800]
Immunohistochemistry-Paraffin: NET1 Antibody [NB100-40800] - (2.5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.Applications for NET1 Antibody
Application
Recommended Usage
Immunohistochemistry
2.5 ug/ml
Immunohistochemistry-Paraffin
2.5 ug/ml
Peptide ELISA
Detection limit 1:16000
Application Notes
WB: Preliminary experiments gave an approx. 80-90 kDa band double band in human placenta lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 67.7 kDa band according to NP_001040625.1. The 80-90 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human breast shows cytoplasm staining of myoepithelial cells of ducts.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NET1
Alternate Names
ARHGEF8neuroepithelial cell-transforming gene 1 protein, guanine nucleotide regulatory protein (oncogene), NET1A, neuroepithelial cell transforming 1, neuroepithelial cell transforming gene 1, neuroepithelioma transforming gene 1, p65 Net1 proto-oncogene protein, Proto-oncogene p65 Net1, Rho guanine nucleotide exchange factor (GEF) 8, Rho guanine nucleotide exchange factor 8, small GTP-binding protein regulator
Gene Symbol
NET1
UniProt
Additional NET1 Products
Product Documents for NET1 Antibody
Product Specific Notices for NET1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...