Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3D4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all NEU-1/Sialidase-1 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 48-315 of human NEU1 (NP_000425)produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

40.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NEU-1/Sialidase-1 Antibody (OTI3D4) - Azide and BSA Free

Western Blot: NEU-1/Sialidase-1 Antibody (OTI3D4)Azide and BSA Free [NBP2-72942]

Western Blot: NEU-1/Sialidase-1 Antibody (OTI3D4)Azide and BSA Free [NBP2-72942]

Western Blot: NEU-1/Sialidase-1 Antibody (OTI3D4) - Azide and BSA Free [NBP2-72942] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NEU1.

Applications for NEU-1/Sialidase-1 Antibody (OTI3D4) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NEU-1/Sialidase-1

The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Neu, a glycoprotein, undergoes transactivation upon heterodimerization with other EGF receptor family members. Neu heterodimerization with ErbB-3 recruits heregulin, which induces phosphoinositide (PI) 3-kinase activation. Activation of Neu potentiates tumor cell motility and protease secretion and invasion, and also modulates cell cycle checkpoint function, DNA repair and apoptotic responses. Amplification and/or overexpression of Neu occurs in 20-30% of breast carcinomas. Measurement of increased Neu expression can be a predictor of disease prognosis. Neu may also prove to be a promising target for therapeutic agents.

Long Name

N-acetyl-alpha-Neuraminidase 1

Alternate Names

NEU1, Sialidase-1

Gene Symbol

NEU1

Additional NEU-1/Sialidase-1 Products

Product Documents for NEU-1/Sialidase-1 Antibody (OTI3D4) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NEU-1/Sialidase-1 Antibody (OTI3D4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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