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NEU2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-21649

Novus Biologicals, part of Bio-Techne
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NBP2-21649

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This affinity purified NEU2 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion near aa 100-125 of Human NEU2. (Uniprot: Q9Y3R4)

Reactivity Notes

This antibody reacts with human NEU2. Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed. Cross-reactivity against Neu1 has not yet been established.

Specificity

This antibody reacts with human NEU2. Based on sequence we expect this antibody to react with neuraminidase from other sources, although specific reactivity has not been confirmed. Cross-reactivity against Neu1 has not yet been established.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for NEU2 Antibody

Western Blot: NEU2 Antibody [NBP2-21649]

Western Blot: NEU2 Antibody [NBP2-21649]

Western Blot: NEU2 Antibody [NBP2-21649] - Affinity Purified anti-Neu2 antibody to detect Neu-2 present in a lysate expressing human Neu2. Molecular weight marker indicates a band of the expected MW. The reactive lower molecular weight band is believed to represent a truncated form of this protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using InfraRed 800 labeled Goat-a-Rabbit IgG (H+L) diluted 1:1,000. InfraRed 800 fluorescence image was captured using the Infrared Imaging System Other detection systems will yield similar results.
Western Blot: NEU2 Antibody [NBP2-21649]

Western Blot: NEU2 Antibody [NBP2-21649]

Western Blot: NEU2 Antibody [NBP2-21649] - Affinity Purified anti-Neu2 antibody to detect recombinant His tagged Neu-2. Molecular weight marker indicates a single band of the expected MW. The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using InfraRed Dye 800 labeled Goat-a-Rabbit IgG [H+L] diluted 1:1000. Other detection systems will yield similar results.
NEU2 Antibody

NEU2 Antibody

Western blot analysis using Immunochemical's Affinity Purified anti-Neu2 antibody to detect recombinant His tagged Neu-2 (1.0 ug loaded). Molecular weight marker (not shown) indicates a single band of the expected MW (43 kDa). The blot was incubated with a 1:500 dilution of the antibody at room temperature for 1 h followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] () diluted 1:1,000. IRDye800 fluorescence image was captured using the Odyssey(R) Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Applications for NEU2 Antibody

Application
Recommended Usage

ELISA

1:10000-1:50000

Western Blot

1:500-1:2000
Application Notes
This product is has been tested by western blot and ELISA and suitable for immunocytochemistry and immunoprecipitation, transfected cell culture, and primary cell culture. A single band of the expected apparent molecular weight (43 kDa) was observed at a 1:500 dilution incubated for 1 h at room temperature. A second lower molecular weight band may represent a truncated form of this protein. Neuraminidase is not very abundant in most tissues and its detection using this antibody may require further optimization. Researchers should determine optimal titers for other applications.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: NEU2

NEU2 belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments.

Long Name

Sialidase-2

Alternate Names

Cytosolic sialidase, EC 3.2.1.18

Gene Symbol

NEU2

UniProt

Additional NEU2 Products

Product Documents for NEU2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NEU2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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