Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Neudesin Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human NENF. The immunogen is located within the last 50 amino acids of NENF. Amino Acid Squence: DGSPNLDFKPEDQPH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Neudesin Antibody - BSA Free

Western Blot: Neudesin AntibodyBSA Free [NBP1-77331]

Western Blot: Neudesin AntibodyBSA Free [NBP1-77331]

Western Blot: Neudesin Antibody [NBP1-77331] - Human kidney tissue lysate with NENF antibody at (A) 1 and (B) 2 ug/mL.
Neudesin Antibody - BSA Free

Western Blot: Rabbit Polyclonal Neudesin Antibody [NBP1-77331]

Western Blot: Rabbit Polyclonal Neudesin Antibody [NBP1-77331] - Analysis of mouse liver tissue lysates diluted with 1X TBST at concentration of 1 ug/ml. Image from a verified customer review.
Neudesin Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Neudesin Antibody - BSA Free [NBP1-77331] -

Immunocytochemistry/ Immunofluorescence: Neudesin Antibody - BSA Free [NBP1-77331] - Immunofluorescence of Neudesin in rat kidney tissue with Neudesin antibody at 20 ug/mL.
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Applications for Neudesin Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Despite its predicted molecular weight of ~20kDa, Neudesin is observed at 37 kDa in SDS-PAGE.

Reviewed Applications

Read 1 review rated 4 using NBP1-77331 in the following applications:

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neudesin

NENF is a secreted protein that is expressed in neurons but not glial cells of the brain. This protein has neurotrophic activity in primary cultured mouse neurons but not mitogenic activity in primary cultured mouse astrocytes. NENF activated mitogen-activated protein (MAP) and phosphotidylinositol-3 kinase pathways and could be inhibited by pertussis toxin but not receptor tyrosine kinases, suggesting that NENF acts through a Gi/Go-protein-coupled receptor. Despite its predicted molecular weight of ~20kDa, NENF is observed at 37kDa in SDS-PAGE.

Alternate Names

CIR2, NENF, SCIRP10, SPUF

Gene Symbol

NENF

UniProt

NP_037481 (Human)

Additional Neudesin Products

Product Documents for Neudesin Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Neudesin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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