Neurochondrin Antibody [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-62175

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Neurochondrin Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to unique epitope on NORB with peptide sequcne corresponding to (EETASHYRMAALEQCLSEP). The NORB peptide was covalently modified post-synthetically to achieve desired antigenicity.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID:25009471)

Specificity

This antibody detects a single band of approximately 88.5kDa in Positive control samples. The antibody also labels a minor 35kDa, the identity of this protein is not known.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Neurochondrin Antibody [FITC]

Application
Recommended Usage

ELISA

< 1:10000

Western Blot

> 1:500
Application Notes
This antibody detects a single band of approximately 88.5kDa in PC- NORB samples. The antibody also labels a minor 35kDa, the identity of this protein is not known. Antibody NORB -101AP is ideal for WB and ELISA applications, other applications have not been tested. These antibodies do not cross react to human lactoferrin, bovine lactoferrin or bovine serum albumin. The species cross reactivity for these antibodies have not been examined fully. The dilutions for this antibody is for reference only, investigators are expected to determine the optimal conditions for specific assay. WB: > 1:500; IMM & i.p pull-down assays: n.d; IHC n.d. ELISA <1:10,000. Application of this antibody in protocols not listed here does not necessarily exclude its use in such procedures.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris/Glycine buffer, pH 7.4-7.8, HEPES,BSA 0.5%, glycerol 30%.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neurochondrin

Norbin, also known as Neurochondrin, is a neuron-specific cytoplasmic protein involved in dendrite outgrowth. Overexpression of norbin in cultured Neuro 2a cells induced neurite-outgrowth (1). Norbin is located at rat chromosome 5q36. In addition to its involvement in dendrite outgrowth, Norbin is implicated in neural plasticity, chondrocyte differentiation, and early embryogenesis and in bone metabolism by inducing bone marrow cells to absorb bone matrix (2). Norbin is also an important endogenous modulator of metabotropic glutamate receptor 5 (mGluR5). It increases the cell surface localization of the receptor and positively regulates its signaling by physically interacting with the receptor (3). Norbin also acts as a signal molecule. It is a negative regulator of Ca2+/Calmodulin-dependent protein kinase II (CaMKII) phosphorylation and it is essential for the spatial learning process of the neurons (4). Recently it was shown that Norbin is a Diaphanous1 (Dia1) interacting protein, specifically binding to only the formin homology 3 (FH3). Furthermore, it was uncovered that the first 100 amino acids, but not the C-terminal region, of Norbin were responsible for its effect on neurite outgrowth (5). Norbin knockout mice showed a behavioral phenotype associated with a rodent model of schizophrenia and disruption of norbin gene resulted in epileptic seizure (3). Targeted disruption of Norbin gene resulted in embryonic lethality (6). It is also suggested that Norbin is involved in age and sex dependent brain function. Norbin is expressed in hippocampus, amygdala, septum, nucleus accumbens and dorsal striatum. It is approximately an 87.5kDa protein (729 amino acids). The NORB-selective antibodies were generated against purified Slingshot1 isoform1 (NORB) protein. NORB-antibodies are affinity purified over immobilized antigen based chromatography, and the purified immunoglobulins are stabilized in antibody stabilization buffer.

Alternate Names

KIAA0607NCDN-1, NCDN-2, neurochondrin

Gene Symbol

NCDN

Additional Neurochondrin Products

Product Documents for Neurochondrin Antibody [FITC]

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Neurochondrin Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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