Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse, C. elegans

Cited:

Nematode - Caenorhabditis elegans

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Neurofibromin 1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2760 and the C-terminus (residue 2818) of human Neurofibromin 1 using the numbering given in entry NP_000258.1 (GeneID 4763)

Reactivity Notes

C. elegans reactivity reported in scientific literature (PMID: 30929290).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Neurofibromin 1 Antibody

Western Blot: Neurofibromin 1 Antibody [NB100-418]

Western Blot: Neurofibromin 1 Antibody [NB100-418]

Western Blot: Neurofibromin 1 Antibody [NB100-418] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Western Blot: Neurofibromin 1 Antibody [NB100-418]

Western Blot: Neurofibromin 1 Antibody [NB100-418]

Western Blot: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 in Western Blot and IP, using NB100-418. Samples: mock treated (control) or NF1-siRNA treated HeLa WCE. IPed NF was detected using an antibody from another vendor.
Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418]

Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418]

Immunoprecipitation: Neurofibromin 1 Antibody [NB100-418] - Detection of human NF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NF1 antibody NB100-418 used for IP at 6 ug per reaction. NF1 was also immunoprecipitated by another rabbit anti-NF1 antibody. For blotting immunoprecipitated NF1, NB100-418 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for Neurofibromin 1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg of lysate

Western Blot

1:1000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30929290).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Neurofibromin 1

Neurofibromin is a product of the tumor suppressor gene, Neurofibromatosis type I. Neurofibromin is known to have GTPase activity that modulates the ras pathway. The absence of or alteration of the neurofibromin protein may lead to Neurofibromatosis disease.

Alternate Names

DKFZp686J1293, FLJ21220, Neurofibromatosis-related protein NF-1, neurofibromin, neurofibromin 1, NFNS, VRNF, WSS

Entrez Gene IDs

4763 (Human)

Gene Symbol

NF1

UniProt

P21359 (Human)

Additional Neurofibromin 1 Products

Product Documents for Neurofibromin 1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neurofibromin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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