Neurofibromin 1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB300-155
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
An N-terminal peptide of Neurofibromin 1 (within amino acids 1-50).
Localization
Cytoplasmic
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Neurofibromin 1 Antibody
![Western Blot: Neurofibromin 1 Antibody [NB300-155]](https://resources.bio-techne.com/images/products/Neurofibromin-1-Antibody-Western-Blot-NB300-155-img0004.jpg "Western Blot: Neurofibromin 1 Antibody [NB300-155]")
Western Blot: Neurofibromin 1 Antibody [NB300-155]
Western Blot: Neurofibromin 1 Antibody [NB300-155] - Western Blot of (A, B, C above).![Immunohistochemistry: Neurofibromin 1 Antibody [NB300-155]](https://resources.bio-techne.com/images/products/Neurofibromin-1-Antibody-Immunohistochemistry-NB300-155-img0003.jpg "Immunohistochemistry: Neurofibromin 1 Antibody [NB300-155]")
Immunohistochemistry: Neurofibromin 1 Antibody [NB300-155]
Immunohistochemistry: Neurofibromin 1 Antibody [NB300-155] - NF1 protein labeling displays positive signal in the maturing and the hypertrophic cartilages but only faint labeling in the proliferative cartilage.Applications for Neurofibromin 1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
reported in scientific literature (PMID 23910086)
Immunohistochemistry
1:200-1:500
Immunohistochemistry-Frozen
1:200-1:500
Immunohistochemistry-Paraffin
1:200-1:500
Western Blot
1:500-1:2000
Application Notes
In Western blot, a band-set at 220-240 kDa and a higher MW band at 450 kDa (uncharacterized) can be seen. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
For IHC, antigen retrieval is not required for immunostaining of frozen sections. Antigen retrieval with trypsin or citrate improves immunostaining of paraffin sections.
For IHC, antigen retrieval is not required for immunostaining of frozen sections. Antigen retrieval with trypsin or citrate improves immunostaining of paraffin sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Whole antisera
Preservative
0.02% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Neurofibromin 1
Alternate Names
DKFZp686J1293, FLJ21220, Neurofibromatosis-related protein NF-1, neurofibromin, neurofibromin 1, NFNS, VRNF, WSS
Gene Symbol
NF1
UniProt
Additional Neurofibromin 1 Products
Product Documents for Neurofibromin 1 Antibody
Product Specific Notices for Neurofibromin 1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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