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Neurofibromin 1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25009

Novus Biologicals, part of Bio-Techne
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NBP3-25009

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein Neurofibromin 1 using the following amino acid sequence: MARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Neurofibromin 1 Antibody

Neurofibromin 1 Antibody Immunocytochemistry/Immunofluorescence: Neurofibromin 1 Antibody [NBP3-25009]

Immunocytochemistry/Immunofluorescence: Neurofibromin 1 Antibody [NBP3-25009]

Staining of human cell line U2OS shows localization to nucleoplasm & plasma membrane.

Applications for Neurofibromin 1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.2, 40% glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Neurofibromin 1

Neurofibromin 1 (NF1) is a suspected negative regulator of the ras signal transduction pathway. Neurofibromin is known to regulate the GTPase HRAS, causing the hydrolyzation and inactivation of GTP. Defects in NF1 lead to Neurofibromatosis disease, Recklinghausen disease or Watson disease. Neurofibromin has not been purified, therefore, most of the information regarding this protein has been deduced from homology analysis of its gene sequence.

Alternate Names

DKFZp686J1293, FLJ21220, Neurofibromatosis-related protein NF-1, neurofibromin, neurofibromin 1, NFNS, VRNF, WSS

Gene Symbol

NF1

Additional Neurofibromin 1 Products

Product Documents for Neurofibromin 1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Neurofibromin 1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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