Neuroligin 1/NLGN1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76348
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Canine
Applications
Immunocytochemistry/ Immunofluorescence, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-TAKQDDPKQQ corresponding to internal region according to NP_055747.1.
Marker
post-Synaptic Marker
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Neuroligin 1/NLGN1 Antibody
![Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348]](https://resources.bio-techne.com/images/products/Neuroligin-1-NLGN1-Antibody-Immunofluorescence-NBP2-76348-img0001.jpg "Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348]")
Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348]
Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348] - Analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).![Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348]](https://resources.bio-techne.com/images/products/Neuroligin-1-NLGN1-Antibody-Immunofluorescence-NBP2-76348-img0002.jpg "Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348]")
Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348]
Immunofluorescence: Neuroligin 1/NLGN1 Antibody [NBP2-76348] - Analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Applications for Neuroligin 1/NLGN1 Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:4000
Application Notes
WB: Preliminary experiments gave an approx. 150 kDa band in human brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 92.0 kDa band according to NP_055747.1. The 150 kDa band was successfully blocked by incubation with the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Neuroligin 1/NLGN1
Alternate Names
Nlg, NLGN1
Gene Symbol
NLGN1
Additional Neuroligin 1/NLGN1 Products
Product Documents for Neuroligin 1/NLGN1 Antibody
Product Specific Notices for Neuroligin 1/NLGN1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...