Neuropeptide B Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05567

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Neuropeptide B Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human Neuropeptide B (NP_683694.1). MARSATLAAAALALCLLLAPPGLAWYKPAAGHSSYSVGRAAGLLSGLRRSPYARRSQPYRGAEPPGGAGASPELQLHPRLRSLAVCVQDVAPNLQRCERLPDGRGTYQCKANVFLSLRAADCLAA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Neuropeptide B Antibody - Azide and BSA Free

Western Blot: Neuropeptide B AntibodyAzide and BSA Free [NBP3-05567]

Western Blot: Neuropeptide B AntibodyAzide and BSA Free [NBP3-05567]

Western Blot: Neuropeptide B Antibody [NBP3-05567] - Western blot analysis of extracts of various cell lines, using Neuropeptide B antibody (NBP3-05567) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: Neuropeptide B Antibody - Azide and BSA Free [NBP3-05567]

Immunocytochemistry/ Immunofluorescence: Neuropeptide B Antibody - Azide and BSA Free [NBP3-05567]

Immunocytochemistry/Immunofluorescence: Neuropeptide B Antibody [NBP3-05567] - Immunofluorescence analysis of NIH/3T3 cells using Neuropeptide B Rabbit pAb (NBP3-05567) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: Neuropeptide B Antibody - Azide and BSA Free [NBP3-05567]

Immunocytochemistry/ Immunofluorescence: Neuropeptide B Antibody - Azide and BSA Free [NBP3-05567]

Immunocytochemistry/Immunofluorescence: Neuropeptide B Antibody [NBP3-05567] - Immunofluorescence analysis of HeLa cells using Neuropeptide B Rabbit pAb (NBP3-05567) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for Neuropeptide B Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS with 50% glycerol, pH7.3.

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Neuropeptide B

Neuropeptide B is a endrogenous ligand of the orphan G protein coupled receptor GPR7. NPB and GPR7 exist in close proximity in the rat hypothalamus, and are ideally positioned to modulate neuroendocrine functions. NPB-immunoreactivity was enriched in many regions within the hypothalamus which also contained high levels of GPR7 mRNA including the ventrormedial hypothalamic nucleus, dorsomedial hypothalamic nucleus, arcuate nucleus, supraoptic retrochiasmatic nucleus, and in the area ventral to the zona incerta.

Alternate Names

L7, neuropeptide B, PPL7, PPNPB, preproneuropeptide B, preproprotein L7

Gene Symbol

NPB

Additional Neuropeptide B Products

Product Documents for Neuropeptide B Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Neuropeptide B Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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