Neuropeptide Y Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46535
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Rabbit, Xenopus
Cited:
Human, Mouse, Rat
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
A synthetic peptide from amino acid region 20-70 of mouse Neuropeptide Y conjugated to an immunogenic carrier protein was used as the immunogen. The antigen is homologous in many other species including human, rat, zebrafish and xenopus.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Neuropeptide Y Antibody
Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535]
Immunohistochemistry: Neuropeptide Y Antibody [NBP1-46535] - Low magnification images showing the double staining pattern of NHE1 with neuropeptide NPY. Scale bar: 100um. Insets: High magnification images showing individual cells with double staining. Scale bar: 10um. Asterisks mark Hoechst-stained nuclei. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-019-42872-w), licensed under a CC-BY license.Immunocytochemistry/ Immunofluorescence: Neuropeptide Y Antibody [NBP1-46535] -
Immunocytochemistry/ Immunofluorescence: Neuropeptide Y Antibody [NBP1-46535] - NHE1 distribution (A) & colocalisation with markers for specific cell types (B–D) & major inputs (E, F, G). (A) NHE1 immunoreactivity is distributed throughout the rostrocaudal axis of the SCN (encircled by the dotted lines). Scale bar: 200 µm. OC: optic chiasm. 3 V: third ventricle. (B–G1) Low magnification images showing the double staining pattern of NHE1 with neuropeptides NP2 (B), GRP (C), & VIP (D) as well as markers for afferent inputs vGluT2 (E), NPY (F), & SERT (G1). Scale bar: 100 µm. Insets: High magnification images showing individual cells with double staining. Scale bar: 10 µm. Asterisks mark Hoechst-stained nuclei. (G2) High magnification image showing high degree of colocalisation (yellow) between NHE1 (green) & SERT (red). Scale bar: 10 µm. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31015514), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Neuropeptide Y Antibody
Application
Recommended Usage
Immunohistochemistry
1:300-1:3000
Immunohistochemistry-Frozen
1:300-1:3000
Immunohistochemistry-Paraffin
1:300-1:3000
Western Blot
1:300-1:3000
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 27612517). Use in IHC-FrFl reported in scientific literature (PMID: 31609204).
Formulation, Preparation, and Storage
Purification
Unpurified
Reconstitution
Reconstitute in 150 ul of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.
Formulation
Lyophilized from whole antisera
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Neuropeptide Y/NPY
Alternate Names
NPY, PYY4
Gene Symbol
NPY
UniProt
Additional Neuropeptide Y/NPY Products
Product Documents for Neuropeptide Y Antibody
Product Specific Notices for Neuropeptide Y Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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