NF-H Antibody (SPM563)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-34380
![Western Blot: NF-H Antibody (SPM563) [NBP2-34380]](https://resources.bio-techne.com/images/products/NF-H-Antibody-SPM563-Western-Blot-NBP2-34380-img0002.jpg "Western Blot: NF-H Antibody (SPM563) [NBP2-34380]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine, Chicken
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SPM563
Concentration
0.2 mg/ml
Product Summary for NF-H Antibody (SPM563)
Immunogen
Human recombinant NF-H protein (Uniprot: P12036)
Localization
Cytoplasmic
Specificity
This monoclonal antibody reacts with a 200kDa protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Marker
Neuronal Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
200 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34427)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for NF-H Antibody (SPM563)
Western Blot: NF-H Antibody (SPM563) [NBP2-34380]
Western Blot: NF-H Antibody (SPM563) [NBP2-34380] - Western Blot Analysis of human brain tissue lysate using NF-H MAb (SPM563).![Immunohistochemistry-Paraffin: NF-H Antibody (SPM563) [NBP2-34380]](https://resources.bio-techne.com/images/products/NF-H-Antibody-SPM563-Immunohistochemistry-Paraffin-NBP2-34380-img0001.jpg "Immunohistochemistry-Paraffin: NF-H Antibody (SPM563) [NBP2-34380]")
Immunohistochemistry-Paraffin: NF-H Antibody (SPM563) [NBP2-34380]
Immunohistochemistry-Paraffin: NF-H Antibody (SPM563) [NBP2-34380] - Formalin-fixed, paraffin-embedded human cerebellum stained with Neurofilament Monoclonal Antibody (SPM563)Applications for NF-H Antibody (SPM563)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunohistochemistry-Frozen
1-2 ug/ml
Immunohistochemistry-Paraffin
0.5-1ug/ml
Western Blot
1-2 ug/ml
Application Notes
ELISA: For coating, order Ab without BSA.
Immunohistochemistry (Frozen & formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Immunohistochemistry (Frozen & formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: NF-H
Antibodies to NF-H may be used to identify NF-H in neurons and to study neuronal processes in tissue sections and/or culture. NF-H antibodies can also be used to study microfilament accumulations seen in many neurological diseases, such as Lou Geri's disease or Alzheimer's disease.
Long Name
Neurofilament Protein, Heavy Chain
Alternate Names
NEFH, NFH
Gene Symbol
NEFH
UniProt
Additional NF-H Products
Product Documents for NF-H Antibody (SPM563)
Product Specific Notices for NF-H Antibody (SPM563)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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