NF2/Merlin Antibody (JE31-02)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32589
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # JE31-02
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human NF2/Merlin aa 546-595 / 595. (Uniprot: P35240)
Localization
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NF2/Merlin Antibody (JE31-02)
Western Blot: NF2/Merlin Antibody (JE31-02) [NBP3-32589]
Western Blot: NF2/Merlin Antibody (JE31-02) [NBP3-32589] - Western blot analysis of NF2/Merlin on different lysates with Rabbit anti-NF2/Merlin antibody (NBP3-32589) at 1/500 dilution.Lane 1: Hela cell lysate (10 ug/Lane)
Lane 2: Mouse brain tissue lysate (20 ug/Lane)
Lane 3: Rat brain tissue lysate (20 ug/Lane)
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32589) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.
Applications for NF2/Merlin Antibody (JE31-02)
Application
Recommended Usage
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NF2/Merlin
Long Name
Neurofibromin 2
Alternate Names
ACN, BANF, Merlin, SCH, Schwannomerlin, Schwannomin
Gene Symbol
NF2
Additional NF2/Merlin Products
Product Documents for NF2/Merlin Antibody (JE31-02)
Product Specific Notices for NF2/Merlin Antibody (JE31-02)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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