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NFATC3/NFAT4 Antibody (3A12)

Novus Biologicals, part of Bio-Techne | Catalog # H00004775-M02

Novus Biologicals, part of Bio-Techne
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H00004775-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 3A12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

NFATC3 (NP_775188, 70 a.a. ~ 149 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. HSSVLSPSFQLQSHKNYEGTCEIPESKYSPLGGPKPFECPSIQITSISPNCHQELDAHEDDLQINDPEREFLERPSRDHL

Specificity

nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 3

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NFATC3/NFAT4 Antibody (3A12)

Immunocytochemistry/ Immunofluorescence: NFATC3/NFAT4 Antibody (3A12) [H00004775-M02]

Immunocytochemistry/ Immunofluorescence: NFATC3/NFAT4 Antibody (3A12) [H00004775-M02]

Immunocytochemistry/Immunofluorescence: NFATC3/NFAT4 Antibody (3A12) [H00004775-M02] - Analysis of monoclonal antibody to NFATC3 on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NFATC3/NFAT4 Antibody (3A12) [H00004775-M02]

Immunohistochemistry-Paraffin: NFATC3/NFAT4 Antibody (3A12) [H00004775-M02]

Immunohistochemistry-Paraffin: NFATC3/NFAT4 Antibody (3A12) [H00004775-M02] - Analysis of monoclonal antibody to NFATC3 on formalin-fixed paraffin-embedded human testis. Antibody concentration 1.5 ug/ml.
ELISA: NFATC3/NFAT4 Antibody (3A12) [H00004775-M02]

ELISA: NFATC3/NFAT4 Antibody (3A12) [H00004775-M02]

ELISA: NFATC3/NFAT4 Antibody (3A12) [H00004775-M02] - Detection limit for recombinant GST tagged NFATC3 is approximately 10ng/ml as a capture antibody.

Applications for NFATC3/NFAT4 Antibody (3A12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
Antibody reactivity against recombinant protein on ELISA. It has been used for IHC-P.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFATC3

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Four transcript variants encoding distinct isoforms have been identified for this gene.

Long Name

Nuclear Factor of Activated T Cells C3

Alternate Names

NFAT4, NFATX

Entrez Gene IDs

4775 (Human)

Gene Symbol

NFATC3

OMIM

602698 (Human)

Additional NFATC3 Products

Product Documents for NFATC3/NFAT4 Antibody (3A12)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NFATC3/NFAT4 Antibody (3A12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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