Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NFATC3 Antibodies »

Product Summary for NFATC3/NFAT4 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1025 and 1075 of human Nuclear Factor of Activated T-cells, Cytoplasmic, Calcineurin-dependent 3 using the numbering given in entry NP_775188.1 (GeneID 4775).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFATC3/NFAT4 Antibody

Western Blot: NFATC3/NFAT4 Antibody [NBP2-04027]

Western Blot: NFATC3/NFAT4 Antibody [NBP2-04027]

Western Blot: NFATC3/NFAT4 Antibody [NBP2-04027] - Detection of human NFATc3 by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug) and HeLa (H; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NFATc3 antibody NBP2-04027 used for WB at 0.1 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 6 ug/mg lysate. NFATc3 was also immunoprecipitated by rabbit anti-NFATc3 antibodies NBP2-04025 and NBP2-04026, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Applications for NFATC3/NFAT4 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 mcg/mg

Western Blot

1:2000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NFATC3

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Four transcript variants encoding distinct isoforms have been identified for this gene.

Long Name

Nuclear Factor of Activated T Cells C3

Alternate Names

NFAT4, NFATX

Gene Symbol

NFATC3

UniProt

NP_775188.1 (Human)

Additional NFATC3 Products

Product Documents for NFATC3/NFAT4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NFATC3/NFAT4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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