NFATC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # H00004776-D01P

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFATC4 Antibodies »

Product Specifications

Immunogen

NFATC4 (NP_004545.2, 1 a.a. - 902 a.a.) full-length human protein. MGAASCEDEELEFKLVFGEEKEAPPLGAGGLGEELDSEDAPPCCRLALGEPPPYGAAPIGIPRPPPPRPGMHSPPPRPAPSPGTWESQPARSVRLGGPGGGAGGAGGGRVLECPSIRITSISPTPEPPAALEDNPDAWGDGSPRDYPPPEGFGGYREAGGQGGGAFFSPSPGSSSLSSWSFFSDASDEAALYAACDEVESELNEAASRFGLGSPLPSPRASPRPWTPEDPWSLYGPSPGGRGPEDSWLLLSAPGPTPASPRPASPCGKRRYSSSGTPSSASPALSRRGSLGEEGSEPPPPPPLPLARDPGSPGPFDYVGAPPAESIPQKTRRTSSEQAVALPRSEEPASCNGKLPLGAEESVAPPGGSRKEVAGMDYLAVPSPLAWSKARIGGHSPIFRTSALPPLDWPLPSQYEQLELRIEVQPRAHHRAHYETEGSRGAVKAAPGGHPVVKLLGYSEKPLTLQMFIGTADERNLRPHAFYQVHRITGKMVATASYEAVVSGTKVLEMTLLPENNMAANIDCAGILKLRNSDIELRKGETDIGRKNTRVRLVFRVHVPQGGGKVVSVQAASVPIECSQRSAQELPQVEAYSPSACSVRGGEELVLTGSNFLPDSKVVFIERGPDGKLQWEEEATVNRLQSNEVTLTLTVPEYSNKRVSRPVQVYFYVSNGRRKRSPTQSFRFLPVICKEEPLPDSSLRGFPSASATPFGTDMDFSPPRPPYPSYPHEDPACETPYLSEGFGYGMPPLYPQTGPPPSYRPGLRMFPETRGTTGCAQPPAVSFLPRPFPSDPYGGRGSSFSLGLPFSPPAPFRPPPLPASPPLEGPFPSQSDVHPLPAEGYNKVGPGYGPGEGAPEQEKSRGGYSSGFRDSVPIQGITLEEVSEIIGRDLSGFPAPPGEEPPA

Specificity

Reacts with nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Quality control test: Antibody reactive against mammalian transfected lysate.

Scientific Data Images for NFATC4 Antibody

Western Blot: NFATC4 Antibody [H00004776-D01P]

Western Blot: NFATC4 Antibody [H00004776-D01P]

Western Blot: NFATC4 Antibody [H00004776-D01P] - Analysis of NFATC4 expression in transfected 293T cell line by NFATC4 polyclonal antibody.Lane 1: NFATC4 transfected lysate(95.40 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NFATC4 Antibody [H00004776-D01P]

Immunocytochemistry/ Immunofluorescence: NFATC4 Antibody [H00004776-D01P]

Immunocytochemistry/Immunofluorescence: NFATC4 Antibody [H00004776-D01P] - Analysis of antibody to NFATC4 on HeLa cell. Antibody concentration 10 ug/ml.
Proximity Ligation Assay: NFATC4 Antibody [H00004776-D01P]

Proximity Ligation Assay: NFATC4 Antibody [H00004776-D01P]

Proximity Ligation Assay: NFATC4 Antibody [H00004776-D01P] - Analysis of protein-protein interactions between NFATC4 and MAPK14. HeLa cells were stained with anti-NFATC4 rabbit purified polyclonal 1:1200 and anti-MAPK14 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for NFATC4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Proximity Ligation Assay

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against transfected lysate in WB and as a detection antibody in ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFATC4

The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family of nuclear factors of activated T cells also participate in the formation of this complex. The product of this gene plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of the IL-2 and IL-4. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq]

Alternate Names

NF-AT3, NFAT3nuclear factor of activated T-cells, cytoplasmic 4, NFATc4, NF-ATc4, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4, T cell transcription factor NFAT3, T-cell transcription factor NFAT3

Entrez Gene IDs

4776 (Human)

Gene Symbol

NFATC4

UniProt

NP_004545.2 (Human)

Additional NFATC4 Products

Product Documents for NFATC4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NFATC4 Antibody

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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