NFATC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46210

Novus Biologicals, part of Bio-Techne
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NBP1-46210
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all NFATC4 Antibodies »

Product Summary for NFATC4 Antibody

Immunogen

The immunogen for this product maps to a region between residue 300 and 350 of human T-cell transcription factor NFAT3 using the numbering given in entry NP_004545.2 (GeneID 4776).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFATC4 Antibody

Western Blot: NFATC4 Antibody [NBP1-46210]

Western Blot: NFATC4 Antibody [NBP1-46210]

Western Blot: NFATC4 Antibody [NBP1-46210] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg), and mouse NIH3T3 (M; 50 mcg) cells. Affinity purified rabbit anti-NFAT3 antibody NBP1-46211 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NFAT3 was also immunoprecipitated by rabbit anti-NFAT3 antibody A302-770A, which recognizes a downstream epitope.
Immunohistochemistry-Paraffin: NFATC4 Antibody [NBP1-46210]

Immunohistochemistry-Paraffin: NFATC4 Antibody [NBP1-46210]

Immunohistochemistry-Paraffin: NFATC4 Antibody [NBP1-46210] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-NFAT3 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB

Applications for NFATC4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NBP1-46210 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NFATC4

Please note, this antibody is considered to be Innovators Grade. Innovators Grade antibodies have been validated for limited uses and require additional characterization for most new species/applications. These antibodies are directed against antigens that have limited or no commercial antibodies available for them, or for which there are limited expression data available to us. In an effort to encourage customer feedback, Novus has made these antibodies available through our Innovators Reward program. Under this program, Novus will provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal value in exchange for your new data. Please contact us at innovators@novusbio.com for more details.

Alternate Names

NF-AT3, NFAT3nuclear factor of activated T-cells, cytoplasmic 4, NFATc4, NF-ATc4, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4, T cell transcription factor NFAT3, T-cell transcription factor NFAT3

Gene Symbol

NFATC4

Additional NFATC4 Products

Product Documents for NFATC4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFATC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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