NFIA Antibody (1E11)
Novus Biologicals, part of Bio-Techne | Catalog # H00004774-M02
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1E11
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
NFIA (AAH22264, 1 a.a. ~ 498 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKEEERAVKDELLSEKPEVKQKWASRLLAKLRKDIRPEYREDFVLTVTGKKPPCCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKSPQCSNPGLCVQPHHIGVSVKELDLYLAYFVHAADSSQSESPSQPSDADIKDQPENGHLGFQDSFVTSGVFSVTELVRVSQTPIAAGTGPNFSLSDLESSSYYSMSPGAMRRSLPSTSSTSSTKRLKSVEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPRASPHATPSTLHFPTSPIIQQPGPYFSHPAIRYHPQETLKEFVQLVCPDAGQQAGQVGFLNPNGSSQGKVHNPFLPTPMLPPPPPPPMARPVPLPVPDTKPPTTSTEGGAASPTSPILVPGIKVAASHHPPDRPPDPFSTL
Specificity
NFIA - nuclear factor I/A (1E11)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for NFIA Antibody (1E11)
![Western Blot: NFIA Antibody (1E11) [H00004774-M02]](https://resources.bio-techne.com/images/products/NFIA-Antibody-1E11-Western-Blot-H00004774-M02-img0005.jpg "Western Blot: NFIA Antibody (1E11) [H00004774-M02]")
Western Blot: NFIA Antibody (1E11) [H00004774-M02]
Western Blot: NFIA Antibody (1E11) [H00004774-M02] - Analysis of NFIA expression in transfected 293T cell line by NFIA monoclonal antibody (M02), clone 1E11.Lane 1: NFIA transfected lysate (Predicted MW: 54.6 KDa).Lane 2: Non-transfected lysate.![Immunocytochemistry/ Immunofluorescence: NFIA Antibody (1E11) [H00004774-M02]](https://resources.bio-techne.com/images/products/NFIA-Antibody-1E11-Immunocytochemistry-Immunofluorescence-H00004774-M02-img0003.jpg "Immunocytochemistry/ Immunofluorescence: NFIA Antibody (1E11) [H00004774-M02]")
Immunocytochemistry/ Immunofluorescence: NFIA Antibody (1E11) [H00004774-M02]
Immunocytochemistry/Immunofluorescence: NFIA Antibody (1E11) [H00004774-M02] - Analysis of monoclonal antibody to NFIA on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: NFIA Antibody (1E11) [H00004774-M02] - Detection limit for recombinant GST tagged NFIA is approximately 0.3ng/ml as a capture antibody.
Applications for NFIA Antibody (1E11)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
Ascites
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NFIA
Alternate Names
CCAAT-box-binding transcription factor, CTF, DKFZp686J23256, FLJ39164, KIAA1439DKFZp434L0422, NF1-A, NF-I/A, NFI-A, NFI-L, nuclear factor 1 A-type, Nuclear factor 1/A, nuclear factor I/ATGGCA-binding protein
Gene Symbol
NFIA
OMIM
600727 (Human)
Additional NFIA Products
Product Documents for NFIA Antibody (1E11)
Product Specific Notices for NFIA Antibody (1E11)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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