NFIA Antibody (PCRP-NFIA-2C6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13982

Novus Biologicals, part of Bio-Techne
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NBP3-13982-100ug
NBP3-13982-20ug
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # PCRP-NFIA-2C6

Concentration

0.2 mg/ml

View all NFIA Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human NFIA protein corresponding to protein domain (Uniprot: Q15784)

Localization

Nucleus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14016)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for NFIA Antibody (PCRP-NFIA-2C6)

Immunocytochemistry/ Immunofluorescence: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982]

Immunocytochemistry/ Immunofluorescence: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982]

Immunocytochemistry/Immunofluorescence: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Immunofluorescence Analysis of PFA-fixed HeLa cells stained using NFIA Antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (green). CF640A phalloidin (red).
Immunohistochemistry-Paraffin: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982]

Immunohistochemistry-Paraffin: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982]

Immunohistochemistry-Paraffin: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Formalin-fixed, paraffin-embedded human colon carcinoma stained with NFIA antibody (PCRP-NFIA-2C6). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.
Flow Cytometry: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982]

Flow Cytometry: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982]

Flow Cytometry: NFIA Antibody (PCRP-NFIA-2C6) [NBP3-13982] - Flow cytometric analysis of PFA-fixed HeLa cells. NFIA antibody (PCRP-NFIA-2C6) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).
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Applications for NFIA Antibody (PCRP-NFIA-2C6)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NFIA

Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.[supplied by OMIM]

Alternate Names

CCAAT-box-binding transcription factor, CTF, DKFZp686J23256, FLJ39164, KIAA1439DKFZp434L0422, NF1-A, NF-I/A, NFI-A, NFI-L, nuclear factor 1 A-type, Nuclear factor 1/A, nuclear factor I/ATGGCA-binding protein

Gene Symbol

NFIA

UniProt

Q15784

Additional NFIA Products

Product Documents for NFIA Antibody (PCRP-NFIA-2C6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFIA Antibody (PCRP-NFIA-2C6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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