NFIA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81407

Novus Biologicals, part of Bio-Techne
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NBP1-81407
NBP1-81407-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFIA Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LKSVEDEMDSPGEEPFYTGQGRSPGSGSQSSGWHEVEPGMPSPTTLKKSEKSGFSSPSPSQTSSLGTAFTQHHRPVITGPRASPHATPSTLHFPTSPIIQQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFIA Antibody

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81407]

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81407]

Immunohistochemistry-Paraffin: NFIA Antibody [NBP1-81407] - Staining of human cerebral cortex, gallbladder, kidney and liver using Anti-PAH antibody (A) NBP1-81407 shows similar protein distribution across tissues to independent antibody NBP1-81406 (B).
Western Blot: NFIA Antibody [NBP1-81407]

Western Blot: NFIA Antibody [NBP1-81407]

Western Blot: NFIA Antibody [NBP1-81407] - Analysis in human cell lines HeLa and Caco-2. Corresponding NFIA RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: NFIA Antibody [NBP1-81407]

Immunocytochemistry/ Immunofluorescence: NFIA Antibody [NBP1-81407]

Immunocytochemistry/Immunofluorescence: NFIA Antibody [NBP1-81407] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
View 5 more images

Applications for NFIA Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFIA

Nuclear factor I (NFI) proteins constitute a family of dimeric DNA-binding proteins with similar, and possibly identical, DNA-binding specificity. They function as cellular transcription factors and as replication factors for adenovirus DNA replication. Diversity in this protein family is generated by multiple genes, differential splicing, and heterodimerization.[supplied by OMIM]

Alternate Names

CCAAT-box-binding transcription factor, CTF, DKFZp686J23256, FLJ39164, KIAA1439DKFZp434L0422, NF1-A, NF-I/A, NFI-A, NFI-L, nuclear factor 1 A-type, Nuclear factor 1/A, nuclear factor I/ATGGCA-binding protein

Gene Symbol

NFIA

Additional NFIA Products

Product Documents for NFIA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFIA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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