NFIC Antibody (1C12-2A2)

Novus Biologicals, part of Bio-Techne | Catalog # H00004782-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C12-2A2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFIC Antibodies »

Product Specifications

Immunogen

NFIC (AAH12120, 1 a.a. ~ 428 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSCRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQHHRPVIAVHSGIARSPHPSSALHFPTTSILPQTASTYFPHTAIRYPPHLNPQDPLKDLVSLACDPASQQPGPSWYLG

Specificity

NFIC - nuclear factor I/C (CCAAT-binding transcription factor)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NFIC Antibody (1C12-2A2)

Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01]

Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01]

Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: NFIC Antibody (1C12-2A2) [H00004782-M01]

Immunocytochemistry/ Immunofluorescence: NFIC Antibody (1C12-2A2) [H00004782-M01]

Immunocytochemistry/Immunofluorescence: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NFIC Antibody (1C12-2A2) [H00004782-M01]

Immunohistochemistry-Paraffin: NFIC Antibody (1C12-2A2) [H00004782-M01]

Immunohistochemistry-Paraffin: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
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Applications for NFIC Antibody (1C12-2A2)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFIC

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication

Alternate Names

CCAAT-binding transcription factor, CTF5, CTFCCAAT-box-binding transcription factor, MGC20153, NF1-C, NFI, NF-I, NF-I/C, NFI-C, nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C, nuclear factor I/C (CCAAT-binding transcription factor), TGGCA-binding protein

Entrez Gene IDs

4782 (Human)

Gene Symbol

NFIC

OMIM

600729 (Human)

UniProt

AAH12120 (Human)

Additional NFIC Products

Product Documents for NFIC Antibody (1C12-2A2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NFIC Antibody (1C12-2A2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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