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NFIC Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19527

Novus Biologicals, part of Bio-Techne
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NBP2-19527

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (98%), Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for NFIC Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human NFIC. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (83%), Chicken (89%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFIC Antibody

Immunohistochemistry-Paraffin: NFIC Antibody [NBP2-19527]

Immunohistochemistry-Paraffin: NFIC Antibody [NBP2-19527]

Immunohistochemistry-Paraffin: NFIC Antibody [NBP2-19527] - Immunohistochemical analysis of paraffin-embedded Breast ca, using antibody at 1:250 dilution.
NFIC Antibody

Western Blot: NFIC Antibody [NBP2-19527] -

Western Blot: NFIC Antibody [NBP2-19527] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with NFIC antibody (NBP2-19527) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for NFIC Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFIC

Recognizes and binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3' present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.

Alternate Names

CCAAT-binding transcription factor, CTF5, CTFCCAAT-box-binding transcription factor, MGC20153, NF1-C, NFI, NF-I, NF-I/C, NFI-C, nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C, nuclear factor I/C (CCAAT-binding transcription factor), TGGCA-binding protein

Gene Symbol

NFIC

UniProt

Additional NFIC Products

Product Documents for NFIC Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NFIC Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.