NFkB p100/p52 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-82063

Novus Biologicals, part of Bio-Techne
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NB100-82063
NB100-82063-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NFkB p100/p52 Antibodies »

Product Specifications

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human NFkB p100/p52 around amino acids 870~874 (S-Q-S-V-E).

Specificity

Detects endogenous levels of total NFkB p100/p52 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFkB p100/p52 Antibody

Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody [NB100-82063] -

Immunocytochemistry/Immunofluorescence: NFkB p100/p52 Antibody [NB100-82063] - Staining of methanol-fixed Hela cells using NB100-82063

Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NB100-82063] -

Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NB100-82063] - Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using NB100-82063 (left) or the same antibody preincubated with blocking peptide (right).

Western Blot: NFkB p100/p52 Antibody [NB100-82063] -

Western Blot: NFkB p100/p52 Antibody [NB100-82063] - Extracts from HT29 cells using NB100-82063 and the same antibody preincubated with blocking peptide

Applications for NFkB p100/p52 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100~1:200

Immunohistochemistry

1:50-1:100

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFkB p100/p52

NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.

Alternate Names

DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Lyt10, LYT10LYT-10, nuclear factor NF-kappa-B p100 subunit, nuclear factor of kappa light chain gene enhancer in B-cells 2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100), Oncogene Lyt-10, p52

Entrez Gene IDs

4791 (Human)

Gene Symbol

NFKB2

UniProt

Q00653

Additional NFkB p100/p52 Products

Product Documents for NFkB p100/p52 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NFkB p100/p52 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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