NFkB p100/p52 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87759

Novus Biologicals, part of Bio-Techne
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NBP1-87759
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFkB p100/p52 Antibodies »

Product Summary for NFkB p100/p52 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ICNYEGPAKIEVDLVTHSDPPRAHAHSLVGKQCSELGICAVSVGPKDMTAQFNNLGVLHVTKKNMMGTMIQKLQRQRLRSRPQGLTEAEQRELEQEAKELKKVMDLSIVRLRFSAFLRASDGSFSLPLKPVISQP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFkB p100/p52 Antibody

Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759]

Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759]

Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining in human appendix and skeletal muscle tissues using anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759]

Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759]

Immunohistochemistry-Paraffin: NFkB p100/p52 Antibody [NBP1-87759] - Staining of human cerebral cortex, kidney, lymph node and testis using Anti-NFKB2 antibody NBP1-87759 (A) shows similar protein distribution across tissues to independent antibody NBP1-87760 (B).
Western Blot: NFkB p100/p52 Antibody [NBP1-87759]

Western Blot: NFkB p100/p52 Antibody [NBP1-87759]

Western Blot: NFkB p100/p52 Antibody [NBP1-87759] - Western blot analysis in human cell lines A-431 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
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Applications for NFkB p100/p52 Antibody

Application
Recommended Usage

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFkB p100/p52

The NFkappaB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkappaB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFkappaB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Alternate Names

DNA-binding factor KBF2, H2TF1, Lymphocyte translocation chromosome 10 protein, Lyt10, LYT10LYT-10, nuclear factor NF-kappa-B p100 subunit, nuclear factor of kappa light chain gene enhancer in B-cells 2, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2, nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100), Oncogene Lyt-10, p52

Gene Symbol

NFKB2

Additional NFkB p100/p52 Products

Product Documents for NFkB p100/p52 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NFkB p100/p52 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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