NFkB p105/p50 Antibody (RM299)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61568

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-61568
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM299

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NFkB p105/p50 Antibodies »

Product Specifications

Immunogen

A peptide corresponding to residues in Human NFkB p105 subunit and p50 subunit.

Reactivity Notes

This antibody may also react to mouse or rat NFkB p105/p50, as predicted by immunogen homology.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFkB p105/p50 Antibody (RM299)

Western Blot: NFkB p105/p50 Antibody (RM299) [NBP2-61568]

Western Blot: NFkB p105/p50 Antibody (RM299) [NBP2-61568]

Western Blot: NFkB p105/p50 Antibody (RM299) [NBP2-61568] - HeLa cell lysate using NBP2-61568.
Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (RM299) [NBP2-61568]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (RM299) [NBP2-61568]

Immunohistochemistry-Paraffin: NFkB p105/p50 Antibody (RM299) [NBP2-61568] - Staining of human tonsil tissue section using NBP2-61568.

Applications for NFkB p105/p50 Antibody (RM299)

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:200

Immunohistochemistry-Paraffin

1:100-1:200

Western Blot

1:100 - 1:200

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFkB p105/p50

NFkB is a transcription regulator that is activated by various intra and extra cellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Serine 529 is located in a negatively charged region (amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth.

Alternate Names

DKFZp686C01211, DNA binding factor KBF1, DNA-binding factor KBF1, EBP-1, KBF1, NF-kappaB, NF-kappa-B, NF-kappabeta, NFKB-p105, NFKB-p50, nuclear factor kappa-B DNA binding subunit, nuclear factor NF-kappa-B p105 subunit, nuclear factor NF-kappa-B p50 subunit, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1MGC54151, p105, p50

Gene Symbol

NFKB1

Additional NFkB p105/p50 Products

Product Documents for NFkB p105/p50 Antibody (RM299)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFkB p105/p50 Antibody (RM299)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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