NFkB2/NFkB p100 [p Ser866] Antibody (1J7E2)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33274

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Mouse

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 1J7E2

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NFkB2 Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S866 of human NFkB2/NFkB p100 (NP_001070962.1).

Sequence:
EDSAY

Modification

p Ser866

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

97 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NFkB2/NFkB p100 [p Ser866] Antibody (1J7E2)

NFkB2/NFkB p100 [p Ser866] Antibody (1J7E2)

Western Blot: NFkB2/NFkB p100 [p Ser866] Antibody (1J7E2) [NBP3-33274] -

Western Blot: NFkB2/NFkB p100 [p Ser866] Antibody (1J7E2) [NBP3-33274] - Western blot analysis of lysates from NIH/3T3 cells, using NFkB2/NFkB p100 Rabbit mAb at 1:500 dilution. NIH/3T3 cells were treated by TNF-alpha (20 ng/ml) at 37C for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

Applications for NFkB2/NFkB p100 [p Ser866] Antibody (1J7E2)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 ug/mL

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFkB2

The NFkappaB transcription factor was originally identified as a protein complex consisting of a DNA binding subunit and an associated protein. The DNA binding subunit is functionally related to c-Rel p75 and Rel B p68. The p50 subunit was initially believed to be a functionally unique protein derived from the amino-terminus of a precursor designated p105. A cDNA has been isolated that encodes an alternative DNA binding subunit of NFkappaB. It is synthesized as a protein that is expressed in a variety of cell types and, like p105, undergoes cleavage to generate its NFkappaB subunit, in this case a protein designated p52 (previously referred to as p49). In contrast to p50 derived from p105, p52 acts in synergy with p65 to stimulate the HIV enhancer in transiently transfected Jurkat cells.

Long Name

Nuclear Factor Kappa-B, Subunit 2

Alternate Names

LYT10, NF-kB2

Gene Symbol

NFKB2

Additional NFkB2 Products

Product Documents for NFkB2/NFkB p100 [p Ser866] Antibody (1J7E2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NFkB2/NFkB p100 [p Ser866] Antibody (1J7E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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