NFS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37933

Novus Biologicals, part of Bio-Techne
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NBP2-37933
NBP2-37933-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (95%), Rat (95%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFS1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GVGAIYIRRRPRVRVEALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINLSF

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFS1 Antibody

Immunocytochemistry/ Immunofluorescence: NFS1 Antibody [NBP2-37933]

Immunocytochemistry/ Immunofluorescence: NFS1 Antibody [NBP2-37933]

Immunocytochemistry/Immunofluorescence: NFS1 Antibody [NBP2-37933] - Staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: NFS1 Antibody [NBP2-37933]

Immunohistochemistry-Paraffin: NFS1 Antibody [NBP2-37933]

Immunohistochemistry-Paraffin: NFS1 Antibody [NBP2-37933] - Staining of human kidney.
Immunohistochemistry: NFS1 Antibody [NBP2-37933]

Immunohistochemistry: NFS1 Antibody [NBP2-37933]

Immunohistochemistry: NFS1 Antibody [NBP2-37933] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
View 4 more images

Applications for NFS1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NFS1

Iron-sulfur clusters are required for the function of many cellular enzymes. The protein encoded by this gene supplies inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process. This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms. Selection of the alternative initiation sites is determined by the cytosolic pH. The encoded protein belongs to the class-V family of pyridoxal phosphate-dependent aminotransferases. [provided by RefSeq]

Alternate Names

cysteine desulfurase, mitochondrial, EC 2.8.1.7, NFS1 nitrogen fixation 1 homolog (S. cerevisiae), NifS, NIFShomolog), nitrogen-fixing bacteria S-like protein

Gene Symbol

NFS1

UniProt

Q9Y697 (Human)

Additional NFS1 Products

Product Documents for NFS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NFS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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