NFX1 Antibody (1D12)

Novus Biologicals, part of Bio-Techne | Catalog # H00004799-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1D12

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NFX1 Antibodies »

Product Specifications

Immunogen

NFX1 (NP_002495, 981 a.a. ~ 1080 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KFSDSLKEDARKDLKFVSDVEKEMETLVEAVNKGKNSKKSHSFPPMNRDHRRIIHDLAQVYGLESVSYDSEPKRNVVVTAIRGKSVCPPTTLTGVLEREM

Localization

Nuclear

Specificity

NFX1 - nuclear transcription factor, X-box binding 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NFX1 Antibody (1D12)

Western Blot: NFX1 Antibody (1D12) [H00004799-M01]

Western Blot: NFX1 Antibody (1D12) [H00004799-M01]

Western Blot: NFX1 Antibody (1D12) [H00004799-M01] - NFX1 monoclonal antibody (M01), clone 1D12 Analysis of NFX1 expression in Hela S3 NE.
Immunocytochemistry/ Immunofluorescence: NFX1 Antibody (1D12) [H00004799-M01]

Immunocytochemistry/ Immunofluorescence: NFX1 Antibody (1D12) [H00004799-M01]

Immunocytochemistry/Immunofluorescence: NFX1 Antibody (1D12) [H00004799-M01] - Analysis of monoclonal antibody to NFX1 on HeLa cell. Antibody concentration 30 ug/ml.
ELISA: NFX1 Antibody (1D12) [H00004799-M01]

ELISA: NFX1 Antibody (1D12) [H00004799-M01]

ELISA: NFX1 Antibody (1D12) [H00004799-M01] - Detection limit for recombinant GST tagged NFX1 is approximately 0.1ng/ml as a capture antibody.

Applications for NFX1 Antibody (1D12)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NFX1

MHC class II gene expression is controlled primarily at the transcriptional level by transcription factors that bind to the X and Y boxes, two highly conserved elements in the proximal promoter of MHC class II genes. The protein encoded by this gene is a transcriptional repressor capable of binding to the conserved X box motif of HLA-DRA and other MHC class II genes in vitro. The protein may play a role in regulating the duration of an inflammatory response by limiting the period in which class II MHC molecules are induced by IFN-gamma. Three alternative splice variants, each of which encodes a different isoform, have been identified.

Alternate Names

DKFZp779G2416, EC 6.3.2.-, MGC20369, NFX2transcriptional repressor NF-X1, Nuclear transcription factor, X box-binding protein 1, nuclear transcription factor, X-box binding 1

Entrez Gene IDs

4799 (Human)

Gene Symbol

NFX1

OMIM

603255 (Human)

Additional NFX1 Products

Product Documents for NFX1 Antibody (1D12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NFX1 Antibody (1D12)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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