NFYC Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93399

Novus Biologicals, part of Bio-Techne
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NBP2-93399-0.1ml
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Key Product Details

Species Reactivity

Validated:

Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NFYC Antibodies »

Product Summary for NFYC Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 27-120 of human NFYC (NP_055038.2). MEEIRNLTVKDFRVQELPLARIKKIMKLDEDVKMISAEAPVLFAKAAQIFITELTLRAWIHTEDNKRRTLQRNDIAMAITKFDQFDFLIDIVPR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NFYC Antibody - BSA Free

Western Blot: NFYC AntibodyBSA Free [NBP2-93399]

Western Blot: NFYC AntibodyBSA Free [NBP2-93399]

Western Blot: NFYC Antibody [NBP2-93399] - Western blot analysis of extracts of various cell lines, using NFYC antibody (NBP2-93399) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Applications for NFYC Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:100 - 1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NFYC

NFYC encodes one subunit of a trimeric complex forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoters of a variety of genes. The encoded protein, subunit C, forms a tight dimer with the B subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. Additional regulation, preliminarily supported by the EST database, may be represented by alternative splicing in this subunit.

Alternate Names

C subunit, nuclear transcription factor Y, gamma

Gene Symbol

NFYC

Additional NFYC Products

Product Documents for NFYC Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NFYC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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