NGDN Antibody (OTI1H4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46162

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1H4

Concentration

1 mg/ml

View all NGDN Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NGDN (NP_001036100) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

35.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NGDN Antibody (OTI1H4)

Western Blot: NGDN Antibody (OTI1H4) [NBP2-46162]

Western Blot: NGDN Antibody (OTI1H4) [NBP2-46162]

Western Blot: NGDN Antibody (1H4) [NBP2-46162] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NGDN.
Immunohistochemistry: NGDN Antibody (OTI1H4) [NBP2-46162]

Immunohistochemistry: NGDN Antibody (OTI1H4) [NBP2-46162]

Immunohistochemistry: NGDN Antibody (1H4) [NBP2-46162] - Analysis of Carcinoma of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: NGDN Antibody (OTI1H4) [NBP2-46162]

Immunohistochemistry: NGDN Antibody (OTI1H4) [NBP2-46162]

Immunohistochemistry: NGDN Antibody (1H4) [NBP2-46162] - Analysis of Human prostate tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Applications for NGDN Antibody (OTI1H4)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NGDN

Neuroguidin is an EIF4E (MIM 133440)-binding protein that interacts with CPEB (MIM 607342) and functions as a translational regulatory protein during development of the vertebrate nervous system (Jung et al., 2006 [PubMed 16705177]).[supplied by OMIM].

Alternate Names

C14orf120, DKFZp564O092, EIF4E-binding protein, eukaryotic initiation factor 4E and CPEB binding protein, LCP5, lpd-2, neuroguidin, neuroguidin, EIF4E binding protein, NGDchromosome 14 open reading frame 120

Gene Symbol

NGDN

Additional NGDN Products

Product Documents for NGDN Antibody (OTI1H4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NGDN Antibody (OTI1H4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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