NGEF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88820

Novus Biologicals, part of Bio-Techne
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NBP1-88820
NBP1-88820-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NGEF Antibodies »

Product Summary for NGEF Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: METRESEDLEKTRRKSASDQWNTDNEPAKVKPELLPEKEETSQADQDIQDKEPHCHIPIKRNSIFNRSIRRKSKAKARDNPERNASCLADSQDNGKSVNEPLTLNIPWSRTPPCRTAMQTDPGAQEMSESSST

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NGEF Antibody

Immunohistochemistry-Paraffin: NGEF Antibody [NBP1-88820]

Immunohistochemistry-Paraffin: NGEF Antibody [NBP1-88820]

Immunohistochemistry-Paraffin: NGEF Antibody [NBP1-88820] - Staining of human Duodenum shows moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: NGEF Antibody [NBP1-88820]

Immunohistochemistry-Paraffin: NGEF Antibody [NBP1-88820]

Immunohistochemistry-Paraffin: NGEF Antibody [NBP1-88820] - Staining of human Cerebellum shows strong cytoplasmic and nuclear positivity in Purkinje cells.
Immunohistochemistry-Paraffin: NGEF Antibody [NBP1-88820]

Immunohistochemistry-Paraffin: NGEF Antibody [NBP1-88820]

Immunohistochemistry-Paraffin: NGEF Antibody [NBP1-88820] - Staining of human Cerebral cortex shows strong cytoplasmic and nuclear positivity in neuronal cells.
View 1 more images

Applications for NGEF Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NGEF

NGEF acts as a guanine nucleotide exchange factor (GEF) which differentially activates the GTPases RHOA, RAC1 and CDC42. Plays a role in axon guidance regulating ephrin-induced growth cone collapse. Upon activation by ephrin through EPHA4, the GEF activity swi

Alternate Names

EPHEXIN, ephexin-1, Eph-interacting exchange protein, neuronal guanine nucleotide exchange factorARHGEF27

Gene Symbol

NGEF

Additional NGEF Products

Product Documents for NGEF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NGEF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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