NGEF Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23338
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat, Chicken
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NGEF synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of mouse NGEF. This sequence has significant homology to the same region in human and rat NGEF, and has low homology to other known proteins. [UniProt# Q8CHT1]
Specificity
The antibody detects an 80 kDa band corresponding to NGEF in Western blots of human A431, Jurkat, and HUVEC cells, as well as rat PC12 cells and mouse brain tissue.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for NGEF Antibody
Western Blot: NGEF Antibody [NBP3-23338] -
Western Blot: NGEF Antibody [NBP3-23338] - Western blot analysis of human A431 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min. (lanes 2 & 4). The blot was probed with anti-NGEF (C-terminal region) (lanes 1 & 2) and anti-NGEF (Tyr-87) (lanes 3 & 4).
Immunocytochemistry/Immunofluorescence: NGEF Antibody [NBP3-23338] -
Immunocytochemistry/Immunofluorescence: NGEF Antibody [NBP3-23338] - Immunocytochemical labeling of phosphorylated Exphexin-1 in pervanadate-treated mouse C2C12. The cells were labeled with rabbit polyclonal NGEF (C-terminal region) and NGEF (Tyr-87) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.Applications for NGEF Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: NGEF
Alternate Names
EPHEXIN, ephexin-1, Eph-interacting exchange protein, neuronal guanine nucleotide exchange factorARHGEF27
Gene Symbol
NGEF
Additional NGEF Products
Product Documents for NGEF Antibody
Product Specific Notices for NGEF Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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