NGFI-B alpha/Nur77/NR4A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-55394

Novus Biologicals, part of Bio-Techne
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NB100-55394
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NGFI-B alpha/Nur77/NR4A1 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GRLPSKPKQPPDAS corresponding to internal region according to NP_775180.1.

Epitope

GRLPSKPKQPPDAS

Specificity

This antibody is expected to recognise isoform a (NP_002126.2 and NP_775180.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for NGFI-B alpha/Nur77/NR4A1 Antibody

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-55394]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-55394]

Western Blot: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-55394] - (2ug/ml) staining of Human Thyroid lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-55394]

Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-55394]

Immunohistochemistry-Paraffin: NGFI-B alpha/Nur77/NR4A1 Antibody [NB100-55394] - (5ug/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NGFI-B alpha/Nur77/NR4A1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1:1000
Application Notes
WB: Approx. 60 kDa band observed in human thyroid gland lysates (calculated MW of 64.5 kDa band according to NP_002126.2 and NP_775180.1).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NGFI-B alpha/Nur77/NR4A1

Nak1, a NR4 Nerve Growth Factor-Like receptor, has been shown to affect the hypothalamic-pituitary axis, neuronal differentiation, neuroendocrine regulation of adrenocortical function, and T-cell apoptosis. Nak1 also is known as nuclear receptor Tr3 and is similar to mouse Nur77 and rat NGFI-B. It is encoded by one of the immediate early genes (IEG) in response to nerve growth factor and other serum growth factors. Nak1 can bind to DNA as a monomer, homodimer, or heterodimer (with retinoid X receptors). Nak1 has been shown to inhibit vascular lesion formation and could serve as a novel target in the treatment of vascular diseases.

Long Name

Nerve Growth Factor-induced Protein I-B alpha/Nuclear Receptor Subfamily 4, Group A, Member 1

Alternate Names

HMR, NAK1, NGFIB alpha, NR4A1, Nur77, TR3

Entrez Gene IDs

3164 (Human); 15370 (Mouse); 79240 (Rat)

Gene Symbol

NR4A1

UniProt

NP_002126.2 (Human)

Additional NGFI-B alpha/Nur77/NR4A1 Products

Product Documents for NGFI-B alpha/Nur77/NR4A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NGFI-B alpha/Nur77/NR4A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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