NGFR/TNFRSF16/p75NTR Antibody (SPM299)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44875

Novus Biologicals, part of Bio-Techne
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NBP2-44875-0.1mg
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Key Product Details

Species Reactivity

Validated:

Human, Baboon, Feline, Ferret, Monkey, Mouse (Negative), Rabbit, Rat (Negative)

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # SPM299

Concentration

0.2 mg/ml

View all NGFR/TNFRSF16 Antibodies »

Product Summary for NGFR/TNFRSF16/p75NTR Antibody (SPM299)

Immunogen

NGFR from A875 melanoma cells

Epitope

aa 1-160

Reactivity Notes

Does not react with Mouse or Rat.

Localization

Cell surface and cytoplasmic

Specificity

It recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

Marker

Soft Tissue Tumor Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-11470)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for NGFR/TNFRSF16/p75NTR Antibody (SPM299)

Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SPM299) [NBP2-44875]

Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SPM299) [NBP2-44875]

Immunohistochemistry-Paraffin: NGFR/TNFRSF16/p75NTR Antibody (SPM299) [NBP2-44875] - Human Melanoma stained with NGFR Monoclonal Antibody (SPM299).

Applications for NGFR/TNFRSF16/p75NTR Antibody (SPM299)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: NGFR/TNFRSF16

p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor. Interacts with NGFRAP1/BEX3. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1. SUBCELLULAR LOCATION: Membrane; single-pass type I membrane protein. DOMAIN: Death domain is responsible for interaction with RANBP9. PTM: N- and O-glycosylated. PTM: Phosphorylated on serine residues. SIMILARITY: Contains 1 death domain. SIMILARITY: Contains 4 TNFR-Cys repeats.

Long Name

Nerve Growth Factor Receptor

Alternate Names

CD271, NGF R, p75 NTR, TNFRSF16

Gene Symbol

NGFR

UniProt

P08138

Additional NGFR/TNFRSF16 Products

Product Documents for NGFR/TNFRSF16/p75NTR Antibody (SPM299)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NGFR/TNFRSF16/p75NTR Antibody (SPM299)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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