NHE1/SLC9A1 Antibody (2F5)

Novus Biologicals, part of Bio-Techne | Catalog # H00006548-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2F5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NHE1/SLC9A1 Antibodies »

Product Specifications

Immunogen

SLC9A1 (AAH12121, 31 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. VLRSHGLQLSPTASTIRSSEPPRERSIGDVTTAPPEVTPESRPVNHSVTDHGMKPRKAFPVLGIDYTHVRTPFEISLWILLACLMKIGFHVIPTISSIVP

Specificity

SLC9A1 - solute carrier family 9 (sodium/hydrogen exchanger), isoform 1 (antiporter, Na+/H+, amiloride sensitive)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for NHE1/SLC9A1 Antibody (2F5)

Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody (2F5) [H00006548-M01]

Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody (2F5) [H00006548-M01]

Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody (2F5) [H00006548-M01] - Analysis of monoclonal antibody to SLC9A1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.
ELISA: NHE1/SLC9A1 Antibody (2F5) [H00006548-M01]

ELISA: NHE1/SLC9A1 Antibody (2F5) [H00006548-M01]

ELISA: NHE1/SLC9A1 Antibody (2F5) [H00006548-M01] - Detection limit for recombinant GST tagged SLC9A1 is approximately 0.3ng/ml as a capture antibody.

Applications for NHE1/SLC9A1 Antibody (2F5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NHE1/SLC9A1

The Na+/H+ antiporter (SLC9A1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others (Mattei et al., 1988 [PubMed 2846238]).[supplied by OMIM]

Long Name

Sodium/Hydrogen Exchanger 1

Alternate Names

APNH, SLC9A1

Entrez Gene IDs

6548 (Human)

Gene Symbol

SLC9A1

OMIM

107310 (Human)

UniProt

AAH12121 (Human)

Additional NHE1/SLC9A1 Products

Product Documents for NHE1/SLC9A1 Antibody (2F5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NHE1/SLC9A1 Antibody (2F5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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