NHE1/SLC9A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38683

Novus Biologicals, part of Bio-Techne
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NBP2-38683
NBP2-38683-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NHE1/SLC9A1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SPESVDLVNEELKGKVLGLSRDPAKVAEEDEDDDGGIMMRSKETSSPGTDDVFTPAPSDSPSSQRIQRCLSDPGPHPEPGEGEPFFPKG

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NHE1/SLC9A1 Antibody

Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683]

Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683]

Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining in human stomach and liver tissues using NBP2-38683 antibody. Corresponding SLC9A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683]

Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683]

Immunohistochemistry-Paraffin: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human kidney, liver, prostate and stomach. (A) shows similar protein distribution across tissues to independent antibody (B).
Immunocytochemistry/ Immunofluorescence: NHE1/SLC9A1 Antibody [NBP2-38683]

Immunocytochemistry/ Immunofluorescence: NHE1/SLC9A1 Antibody [NBP2-38683]

Immunocytochemistry/Immunofluorescence: NHE1/SLC9A1 Antibody [NBP2-38683] - Staining of human cell line A-431 shows localization to plasma membrane. Antibody staining is shown in green.
View 4 more images

Applications for NHE1/SLC9A1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10 - 1:500

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NHE1/SLC9A1

The Na+/H+ antiporter (SLC9A1) is a ubiquitous membrane-bound enzyme involved in pH regulation of vertebrate cells. It is specifically inhibited by the diuretic drug amiloride and activated by a variety of signals including growth factors, mitogens, neurotransmitters, tumor promoters, and others (Mattei et al., 1988 [PubMed 2846238]).[supplied by OMIM]

Long Name

Sodium/Hydrogen Exchanger 1

Alternate Names

APNH, SLC9A1

Gene Symbol

SLC9A1

UniProt

P19634 (Human)

Additional NHE1/SLC9A1 Products

Product Documents for NHE1/SLC9A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NHE1/SLC9A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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