NHE3/SLC9A3 [p Ser605] Antibody (10A8)

Novus Biologicals, part of Bio-Techne | Catalog # NB110-74678

Novus Biologicals, part of Bio-Techne
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NB110-74678
NB110-74678SS
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat, Porcine, Opossum, Primate, Rabbit

Cited:

Rat, Porcine, Opossum, Primate

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot

Cited:

SDS-Page, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 10A8

Concentration

2.1 mg/ml

View all NHE3/SLC9A3 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to a region surrounding the Serine 605 portion of rat NHE3. [UniProt# P26433]

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID: 29510506).

Modification

p Ser605

Localization

Membrane.

Specificity

This antibody detects NHE3 posphorylated at serine 605, and does not cross-react with the non-phosphopeptide.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NHE3/SLC9A3 [p Ser605] Antibody (10A8)

Western Blot: NHE3/SLC9A3 [p Ser605] Antibody (10A8) [NB110-74678] - Detection of phospho Ser605 in transfected COS-7 cells using NB110-74678. A. untreated B. forskolin and IBMX treated.

Applications for NHE3/SLC9A3 [p Ser605] Antibody (10A8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20

Immunoprecipitation

1:10-1:500

Western Blot

1:1000
Application Notes
This NHE3 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunoprecipitation. In WB a band can be seen at ~85 kDa.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

2.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NHE3/SLC9A3

Na+/H+ exchanger (NHE) family members are integral membrane proteins which play key role in catalyzing the extrusion of intracellular proton (H+) ions in exchange for extracellular sodium (Na+) ions for regulation of cellular pH as well as transepithelial ion/water transport. NHE3 or SLC9A3 is one of the best characterized NHEs that is a major proton extruding system driven by inward Na+ ion chemical gradient and is implicated in pH regulation to eliminate acids generated during metabolism or to tackle any potential adverse conditions in tissue microenvironment. NHE3 exists as a multi-pass membrane protein in cell membranes and is expressed most abundantly in colon and small intestine, followed by kidney and stomach, as well as in ventrolateral region of the medulla oblongata. In intestinal epithelial cells, it localizes to the ileal brush border and SGK1 mediated phosphorylation at Ser-661 has been shown to be associated with its enhanced cell membrane localization. NHE3 binds to SLC9A3R1 and SLC9A3R2, and interacts with CHP1, CHP2, SHANK2 PDZD3 and (interactions may decrease in response to elevated calcium ion levels). NHE3 is regulated by several hormones, neurotransmitters, and associated signaling systems such as cAMP, cGMP, and elevated intracellular calcium via mechanisms that are largely partially understood. NHE3 knock-out mice suffers from chronic diarrhea and altered salt as well as water homeostasis.

Long Name

Sodium/Hydrogen Exchanger 3

Alternate Names

SLC9A3

Gene Symbol

SLC9A3

Additional NHE3/SLC9A3 Products

Product Documents for NHE3/SLC9A3 [p Ser605] Antibody (10A8)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NHE3/SLC9A3 [p Ser605] Antibody (10A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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