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NHE3/SLC9A3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46581

Novus Biologicals, part of Bio-Techne
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NBP1-46581
NBP1-46581-0.025ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Product Specifications

Immunogen

A synthetic peptide from amino acid region 650-700 of human NHE3/SLC9A3 conjugated to blue carrier protein was used as the antigen.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for NHE3/SLC9A3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:200-1:2000

Immunohistochemistry-Frozen

1:200-1:2000

Western Blot

1:200-1:2000
Application Notes
The optimal dilution should be determined by the end user. Although not tested this antibody may be useful in Immunohistochemistry-Paraffin. ICC/IF, and IHC-FR were reported in scientific literature (PMID: 23548792).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NHE3/SLC9A3

NHE3 is a member of the Sodium/Hydrogen exchanger family that plays a crucial role in acid-base and volume homeostasis by mediating the majority of sodium and bicarbonate reabsorption in the proximal tubule of the kidney. Two PKA consensus sites have been discovered in rat NHE3, serine 552 and serine 605. Research suggests that dopamine-induced phosphorylation of these consensus sites inhibit NHE3.

Long Name

Sodium/Hydrogen Exchanger 3

Alternate Names

SLC9A3

Entrez Gene IDs

6550 (Human); 24784 (Rat)

Gene Symbol

SLC9A3

UniProt

Additional NHE3/SLC9A3 Products

Product Documents for NHE3/SLC9A3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NHE3/SLC9A3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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