NHE3/SLC9A3 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00589
![Western Blot: NHE3/SLC9A3 AntibodyBSA Free [NBP3-00589]](https://resources.bio-techne.com/images/products/NHE3-SLC9A3-Antibody-Western-Blot-NBP3-00589-img0003.jpg "Western Blot: NHE3/SLC9A3 AntibodyBSA Free [NBP3-00589]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Partial synthetic peptide made to an internal portion of the human NHE3 protein (between amino acids 600-660) [UniProt P48764]
Clonality
Polyclonal
Host
Rabbit
Scientific Data Images for NHE3/SLC9A3 Antibody - BSA Free
Western Blot: NHE3/SLC9A3 AntibodyBSA Free [NBP3-00589]
Western Blot: NHE3/SLC9A3 Antibody [NBP3-00589] - Total protein from human Hek293 and HeLa cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NHE3 (NBP3-00589) in blocking buffer and detected with an anti-rabbit HRP secondary antibody using NovaLume chemiluminescence detection reagent (NPB2-61915).![Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody - BSA Free [NBP3-00589]](https://resources.bio-techne.com/images/products/NHE3-SLC9A3-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-00589-img0002.jpg "Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody - BSA Free [NBP3-00589]")
Immunocytochemistry/ Immunofluorescence: NHE3/SLC9A3 Antibody - BSA Free [NBP3-00589]
Immunocytochemistry/Immunofluorescence: NHE3/SLC9A3 Antibody [NBP3-00589] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-NHE3/SLC9A3 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.![Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody - BSA Free [NBP3-00589]](https://resources.bio-techne.com/images/products/NHE3-SLC9A3-Antibody-Immunohistochemistry-Paraffin-NBP3-00589-img0001.jpg "Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody - BSA Free [NBP3-00589]")
Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody - BSA Free [NBP3-00589]
Immunohistochemistry-Paraffin: NHE3/SLC9A3 Antibody [NBP3-00589] - Analysis of a FFPE tissue section of human small intestine using 1:200 dilution of NHE3 antibody. The staining was developed using HRP labeled anti-rabbit secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This NHE3 antibody generated nuclear staining.Applications for NHE3/SLC9A3 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
5 - 10 ug/ml
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Western Blot
2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: NHE3/SLC9A3
Long Name
Sodium/Hydrogen Exchanger 3
Alternate Names
SLC9A3
Gene Symbol
SLC9A3
Additional NHE3/SLC9A3 Products
Product Documents for NHE3/SLC9A3 Antibody - BSA Free
Product Specific Notices for NHE3/SLC9A3 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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