NHERF-1 Antibody (EBP-10) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB500-488
![NHERF-1 Antibody (EBP-10) - BSA Free Western Blot: NHERF-1 Antibody (EBP-10) - BSA Free [NB500-488]](https://resources.bio-techne.com/images/products/nb500-488_mouse-monoclonal-nherf-1-antibody-ebp-10-1312024169400.jpg "Western Blot: NHERF-1 Antibody (EBP-10) - BSA Free [NB500-488]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # EBP-10
Format
BSA Free
Concentration
1.0 mg/ml
Product Summary for NHERF-1 Antibody (EBP-10) - BSA Free
Immunogen
Bacterially produced recombinant full-length human NHERF1.
Specificity
The antibody EBP-10 reacts with NHERF1/EBP50 phosphoprotein of 50 kDa, which serves as an adaptor and regulator protein.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NHERF-1 Antibody (EBP-10) - BSA Free
Western Blot: NHERF-1 Antibody (EBP-10) - BSA Free [NB500-488]
Analysis of human NHERF1 using mouse monoclonal antibody EBP-10 on lysates of Jurkat, MCF-7, and Daudi cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-NHERF1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. NHERF1 was detected around 50 kDa.Applications for NHERF-1 Antibody (EBP-10) - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10 - 1:500
Immunohistochemistry-Paraffin
5 ug/ml
Immunoprecipitation
1:50
Western Blot
2 ug/ml
Application Notes
Western blotting: Recommended dilution: 2 ug/ml, reducing conditions, positive control: MCF-7 cell line.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.4)
Format
BSA Free
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NHERF-1
Alternate Names
EBP50NHERF-1, ezrin-radixin-moesin binding phosphoprotein-50, Ezrin-radixin-moesin-binding phosphoprotein 50, Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Na+/H+ exchange regulatory co-factor, NHERF1solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulator 1, NHERFNPHLOP2, Regulatory cofactor of Na(+)/H(+) exchanger, sodium/hydrogen exchanger regulatory factor 1, Sodium-hydrogen exchanger regulatory factor 1, solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 regulatoryfactor 1, solute carrier family 9 (sodium/hydrogen exchanger), member 3 regulator 1, Solute carrier family 9 isoform A3 regulatory factor 1
Entrez Gene IDs
9368 (Human)
Gene Symbol
NHERF1
Additional NHERF-1 Products
Product Documents for NHERF-1 Antibody (EBP-10) - BSA Free
Product Specific Notices for NHERF-1 Antibody (EBP-10) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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