NHLH1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93392
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-60 of human NHLH1 (NP_005589.1). MMLNSDTMELDLPPTHSETESGFSDCGGGAGPDGAGPGGPGGGQARGPEPGEPGRKDLQH
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NHLH1 Antibody - Azide and BSA Free
Immunoprecipitation: NHLH1 Antibody - Azide and BSA Free [NBP2-93392] -
Immunoprecipitation: NHLH1 Antibody - Azide and BSA Free [NBP2-93392] - Immunoprecipitation analysis of 300ug extracts of 293F-NHLH1-Avi-His(C) cells using 3ug NHLH1 Rabbit pAb . Western blot was performed from the immunoprecipitate using NHLH1 Rabbit pAb antibody at a dilition of 1:3000.
Western Blot: NHLH1 Antibody - Azide and BSA Free [NBP2-93392] -
Western Blot: NHLH1 Antibody - Azide and BSA Free [NBP2-93392] - Western blot analysis of lysates from wild type (WT) and 293T cells transfected with NHLH1 using NHLH1 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: NHLH1 Antibody - Azide and BSA Free [NBP2-93392] -
Immunocytochemistry/ Immunofluorescence: NHLH1 Antibody - Azide and BSA Free [NBP2-93392] - Immunofluorescence analysis of 293F cells transfected with NHLH1 using NHLH1 Rabbit pAb at a dilution of 1:400 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.Applications for NHLH1 Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NHLH1
Alternate Names
BHLHA35, bHLHa35HEN-1, helix-loop-helix protein 1, HEN1NSCL-1, nescient helix loop helix 1Class A basic helix-loop-helix protein 35, NSCL, NSCL1
Gene Symbol
NHLH1
Additional NHLH1 Products
Product Documents for NHLH1 Antibody - Azide and BSA Free
Product Specific Notices for NHLH1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov