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NHP2L1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32732

Novus Biologicals, part of Bio-Techne
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NBP1-32732

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Whole-Mount, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Full length human NHP2L1 Recombinant protein.

Localization

Nucleus, nucleolus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

14 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NHP2L1 Antibody

Western Blot: NHP2L1 Antibody [NBP1-32732]

Western Blot: NHP2L1 Antibody [NBP1-32732]

Western Blot: NHP2L1 Antibody [NBP1-32732] - A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat2 whole cell lysate/extract15 % SDS-PAGE NHP2L1 antibody dilution: 1:1000
Immunocytochemistry/ Immunofluorescence: NHP2L1 Antibody [NBP1-32732]

Immunocytochemistry/ Immunofluorescence: NHP2L1 Antibody [NBP1-32732]

Immunocytochemistry/Immunofluorescence: NHP2L1 Antibody [NBP1-32732] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732]

Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732]

Immunohistochemistry-Paraffin: NHP2L1 Antibody [NBP1-32732] - Paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.

Applications for NHP2L1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID: 26106861). IHC-Wm Assay dependent.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NHP2L1

Originally named because of its sequence similarity to the Saccharomyces cerevisiae NHP2 (non-histone protein 2), this protein appears to be a highly conserved nuclear protein that is a component of the [U4/U6.U5] tri-snRNP. It binds to the 5' stem-loop of U4 snRNA. Two transcript variants encoding the same protein have been found for this gene.

Alternate Names

[U4/U6.U5] tri-snRNP 15.5 kD RNA binding protein, 15.5K, FA1, FA-1, High mobility group-like nuclear protein 2 homolog 1, hSNU13, NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae), NHP2-like protein 1, NHPX, non-histone chromosome protein 2 (S. cerevisiae)-like 1, non-histone chromosome protein 2-like 1, OTK27, small nuclear ribonucleoprotein 15.5kDa (U4/U6.U5), SNRNP15-5, SNU13, SNU13 homolog, SPAG12, sperm specific antigen 1, SSFA1, U4/U6.U5 tri-snRNP 15.5 kDa protein

Entrez Gene IDs

4809 (Human); 20826 (Mouse); 300092 (Rat)

Gene Symbol

SNU13

UniProt

Additional NHP2L1 Products

Product Documents for NHP2L1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NHP2L1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.