Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-61673

Novus Biologicals, part of Bio-Techne
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NBP2-61673
NBP2-61673-0.025ml
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6D3A10

Format

BSA Free

Concentration

1 mg/ml

View all Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human Nicotinic Acetylcholine R alpha 3/CHRNA3 (AA: 32-240) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

57.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free

Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10)BSA Free [NBP2-61673]

Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10)BSA Free [NBP2-61673]

Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis using CHRNA3 mAb against human CHRNA3 (AA: 32-240) recombinant protein. (Expected MW is 50.6 kDa)
Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free [NBP2-61673]

Immunocytochemistry/ Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free [NBP2-61673]

Immunocytochemistry/Immunofluorescence: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Goat anti-Mouse IgG (H+L) DyLight 488 secondary antibody was used.
Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free [NBP2-61673]

Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free [NBP2-61673]

Flow Cytometry: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) [NBP2-61673] - Analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red).
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Applications for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200-1:400

Immunocytochemistry/ Immunofluorescence

1:200-1:1000

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 3/CHRNA3

This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described.

Long Name

Nicotinic Acetylcholine Receptor alpha 3

Alternate Names

CHRNA3, LNCR2, NACHRA3, Nicotinic Acetylcholine Ra3, PAOD2

Gene Symbol

CHRNA3

Additional Nicotinic Acetylcholine R alpha 3/CHRNA3 Products

Product Documents for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody (6D3A10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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