Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94776

Novus Biologicals, part of Bio-Techne
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NBP2-94776-0.02ml
NBP2-94776-0.1ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 32-240 of human CHRNA3 (NP_000734.2). SEAEHRLFERLFEDYNEIIRPVANVSDPVIIHFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWNPSDYGGAEFMRVPAQKIWKPDIVLYNNAVGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHDIKYNCCEEIYPDITYSLYIRRL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody - BSA Free

Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 AntibodyBSA Free [NBP2-94776]

Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 AntibodyBSA Free [NBP2-94776]

Western Blot: Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody [NBP2-94776] - Western blot analysis of extracts of various cell lines, using Nicotinic Acetylcholine R alpha 3/CHRNA3 antibody (NBP2-94776) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R alpha 3/CHRNA3

The nicotinic acetylcholine receptor (nAChR) is a ligand gated ion channel that mediates neurotransmission at the neuromuscular junction, autonomic ganglia and at some sites in the central nervous system. Distinct nAChR subtypes exist that can be stimulated by the neurotransmitter acetylcholine, the natural product nicotine, or by synthetic compounds. After binding acetylcholine, Nicotinic Acetylcholine Receptor alpha 3 responds by an extensive change in conformation that affects all subunits and leads to opening of an ion conducting channel across the plasma membrane.

Long Name

Nicotinic Acetylcholine Receptor alpha 3

Alternate Names

CHRNA3, LNCR2, NACHRA3, Nicotinic Acetylcholine Ra3, PAOD2

Gene Symbol

CHRNA3

Additional Nicotinic Acetylcholine R alpha 3/CHRNA3 Products

Product Documents for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nicotinic Acetylcholine R alpha 3/CHRNA3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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