Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3)
Novus Biologicals, part of Bio-Techne | Catalog # H00001138-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 7D3
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
CHRNA5 (NP_000736, 38 a.a. ~ 131 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SEPSSIAKHEDSLLKDLFQDYERWVRPVEHLNDKIKIKFGLAISQLVDVDEKNQLMTTNVWLKQEWIDVKLRWNPDDYGGIKVIRVPSDSVWTP
Specificity
CHRNA5 - cholinergic receptor, nicotinic, alpha polypeptide 5
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3)
![Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3) [H00001138-M01]](https://resources.bio-techne.com/images/products/Nicotinic-Acetylcholine-R-alpha-5-CHRNA5-Antibody-7D3-Western-Blot-H00001138-M01-img0002.jpg "Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3) [H00001138-M01]")
Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3) [H00001138-M01]
Western Blot: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3) [H00001138-M01] - Analysis of CHRNA5 expression in transfected 293T cell line by CHRNA5 monoclonal antibody (M01), clone 7D3.Lane 1: CHRNA5 transfected lysate (Predicted MW: 53.1 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3) [H00001138-M01] - Detection limit for recombinant GST tagged CHRNA5 is 0.3 ng/ml as a capture antibody.
Applications for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Nicotinic Acetylcholine R alpha 5/CHRNA5
Long Name
Nicotinic Acetylcholine Receptor alpha 5
Alternate Names
CHRNA5, NACHRA5, Nicotinic Acetylcholine Ra5
Entrez Gene IDs
1138 (Human)
Gene Symbol
CHRNA5
OMIM
118505 (Human)
UniProt
Additional Nicotinic Acetylcholine R alpha 5/CHRNA5 Products
Product Documents for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3)
Product Specific Notices for Nicotinic Acetylcholine R alpha 5/CHRNA5 Antibody (7D3)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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