Nicotinic Acetylcholine Receptor beta 4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-20901

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Nicotinic Acetylcholine R beta 4/CHRNB4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-DYRLTWNSSRYEGVN corresponding to internal region according to NP_000741.1.

Epitope

C terminus

Reactivity Notes

Expected from sequence similarity: Bovine

Specificity

The immunizing peptide represents part of the first exrracellular domain (aa22-236).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Nicotinic Acetylcholine Receptor beta 4 Antibody

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine Receptor beta 4 Antibody [NBP1-20901]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine Receptor beta 4 Antibody [NBP1-20901]

Immunohistochemistry-Paraffin: Nicotinic Acetylcholine Receptor beta 4 Antibody [NBP1-20901] - (2.5ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Nicotinic Acetylcholine Receptor beta 4 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

2.5 ug/ml

Peptide ELISA

Detection limit 1:16000

Western Blot

0.2 ug/ml
Application Notes
Western blot: Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: Paraffin embedded Human Uterus.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R beta 4/CHRNB4

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero) pentamers composed of homologous subunits. After binding acetylcholine, the nAChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Long Name

Nicotinic Acetylcholine Receptor beta 4

Alternate Names

CHRNB4, NAChRB4

Entrez Gene IDs

1143 (Human)

Gene Symbol

CHRNB4

UniProt

NP_000741.1 (Human)

Additional Nicotinic Acetylcholine R beta 4/CHRNB4 Products

Product Documents for Nicotinic Acetylcholine Receptor beta 4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nicotinic Acetylcholine Receptor beta 4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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