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Nicotinic Acetylcholine Receptor epsilon Antibody (1H5)

Novus Biologicals, part of Bio-Techne | Catalog # H00001145-M02

Novus Biologicals, part of Bio-Techne
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H00001145-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 1H5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

CHRNE (NP_000071, 21 a.a. ~ 130 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. KNEELRLYHHLFNNYDPGSRPVREPEDTVTISLKVTLTNLISLNEKEETLTTSVWIGIDWQDYRLNYSKDDFGGIETLRVPSELVWLPEIVLENNIDGQFGVAYDANVLV

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Nicotinic Acetylcholine Receptor epsilon Antibody (1H5)

Sandwich ELISA: Nicotinic Acetylcholine Receptor epsilon Antibody (1H5) [H00001145-M02] - Detection limit for recombinant GST tagged CHRNE is 0.3 ng/ml as a capture antibody.

Applications for Nicotinic Acetylcholine Receptor epsilon Antibody (1H5)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nicotinic Acetylcholine R epsilon/CHRNE

Acetylcholine receptors at mature mammalian neuromuscular junctions are pentameric protein complexes composed of four subunits in the ratio of two alpha subunits to one beta, one epsilon, and one delta subunit. The achetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. Mutations in the epsilon subunit are associated with congenital myasthenic syndrome.

Long Name

Nicotinic Acetylcholine Receptor epsilon

Alternate Names

ACHRE, CHRNE, nAChR epsilon

Gene Symbol

CHRNE

UniProt

Additional Nicotinic Acetylcholine R epsilon/CHRNE Products

Product Documents for Nicotinic Acetylcholine Receptor epsilon Antibody (1H5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nicotinic Acetylcholine Receptor epsilon Antibody (1H5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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