Nidogen-1/Entactin Antibody (ELM1) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97701
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # ELM1
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Derived by fusion of X63 Ag 8.653 mouse myeloma cells with spleen cells from a Fisher rat immunized with a partially purified preparation of laminin from the EHS mouse tumor.
Specificity
This antibody reacts with Nidogen-1/Entactin.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Description
The antibody may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Applications for Nidogen-1/Entactin Antibody (ELM1) - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:25-1:200
Western Blot
1:100-1:1000
Application Notes
This antibody is suitable for immunoblotting, immunocytochemistry and immunohistochemistry on frozen tissues. Optimal antibody dilution should be determined by titration; recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications..
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
PBS, pH 7.2
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Nidogen-1/Entactin
Alternate Names
Entactin-1, NID1, Nidogen1
Gene Symbol
NID1
Additional Nidogen-1/Entactin Products
Product Documents for Nidogen-1/Entactin Antibody (ELM1) - BSA Free
Product Specific Notices for Nidogen-1/Entactin Antibody (ELM1) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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