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Nidogen-1/Entactin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16341

Novus Biologicals, part of Bio-Techne
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NBP2-16341

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nidogen-1/Entactin. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (80%), Bovine (80%).

Localization

Secreted; extracellular space; extracellular matrix; basement membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

136 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nidogen-1/Entactin Antibody

Western Blot: Nidogen-1/Entactin Antibody [NBP2-16341]

Western Blot: Nidogen-1/Entactin Antibody [NBP2-16341]

Western Blot: Nidogen-1/Entactin Antibody [NBP2-16341] - Sample (30 ug of whole cell lysate) A: NT2D1 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunohistochemistry-Paraffin: Nidogen-1/Entactin Antibody [NBP2-16341]

Immunohistochemistry-Paraffin: Nidogen-1/Entactin Antibody [NBP2-16341]

Immunohistochemistry-Paraffin: Nidogen-1/Entactin Antibody [NBP2-16341] - Human liver carcinoma, using Nidogen antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for Nidogen-1/Entactin Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nidogen-1/Entactin

This gene encodes a member of the nidogen family of basement membrane glycoproteins. The protein interacts with several other components of basement membranes, and may play a role in cell interactions with the extracellular matrix. [provided by RefSeq]

Alternate Names

Entactin-1, NID1, Nidogen1

Gene Symbol

NID1

UniProt

Additional Nidogen-1/Entactin Products

Product Documents for Nidogen-1/Entactin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nidogen-1/Entactin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.