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Niemann-Pick type C1 Like-1 Antibody (4B10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15211

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15211

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4B10

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for Niemann-Pick type C1 Like-1 Antibody (4B10)

Immunogen

A synthesized peptide derived from human Niemann-Pick type C1 Like-1. UniProt ID (Q9UHC9).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Niemann-Pick type C1 Like-1 Antibody (4B10)

Immunohistochemistry: Niemann-Pick type C1 Like-1 Antibody (4B10) [NBP3-15211]

Immunohistochemistry: Niemann-Pick type C1 Like-1 Antibody (4B10) [NBP3-15211]

Immunohistochemistry: Niemann-Pick type C1 Like-1 Antibody (4B10) [NBP3-15211] - IHC image of Niemann-Pick type C1 Like-1 antibody diluted at 1:100 and staining in paraffin-embedded human liver tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for Niemann-Pick type C1 Like-1 Antibody (4B10)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: Niemann-Pick type C1 Like-1

Dietary consumption and intestinal cholesterol absorption contribute to plasma cholesterol levels, which factor into the risk of coronary heart disease. Niemann-Pick type C1 Like 1 protein (NPC1L1 or NPC3) is enriched in the small intestine and is found in the brush border membrane of enterocytes. It plays a critical role in absorption of intestinal cholesterol.

Alternate Names

Niemann-Pick C1-like protein 1, NPC1 (Niemann-Pick disease, type C1, gene)-like 1, NPC11L1

Gene Symbol

NPC1L1

Additional Niemann-Pick type C1 Like-1 Products

Product Documents for Niemann-Pick type C1 Like-1 Antibody (4B10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Niemann-Pick type C1 Like-1 Antibody (4B10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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